NB 1-3 Details
NB 1-3:
This precursor has not been included in early NB maps (Doe, 1992; Broadus et al., 1995).
It was first identified by Schmidt et al. (1997) as "NB 1-3," an extremely lateral huckebein-positive cell that produces glial cells and 3 motoneurons that project contralaterally into both the ISN and SN, and they observed one of these MNs to innervate muscle 14.
We have generated one clone similar to "NB 1-3" (we usually did not label at extremely lateral positions), and we believe it produces some of the glia of the transverse nerve (TN), rather than motoneurons. The TN contains neurosecretory cells, motoneurons, mesodermally-derived "DM cells", glia, and sensory axons (Fig. 1-3, inset). In Drosophila, the origin of the TN glia is unclear, but in Manduca, the TN glia (including the "strap cells") develop from a lateral position similar to NB 1-3 (Carr and Taghert; 1988a,). Our clone contains cells that closely match those described for Manduca TN glia, and hence we propose that they are Drosophila TN glia. We also detected a PNS subclone in this lineage (not shown). [http://www.neuro.uoregon.edu/doelab/lineages/NB1-3.html]
We labeled the mesodermal precursor of the Transverse Nerve DM cells and found that lineage to include the muscle 6,7,12 and 13 progenitors as well (n=6, Fig. 1-3).
References:
Broadus, J., Skeath, J.B., Spana, E. P., Bossing, T., Technau, G.M., and Doe, C.Q. (1995). New neuroblast markers and the origin of the aCC/pCC neurons in the Drosophila central nervous system. Mech Dev 53: 393-402.
Carr, J.N., and Taghert, P. H. (1988a). Formation of the Transverse Nerve in moth embryos: I. A scaffold of non-neuronal cells prefigures the nerve. Dev Biol 130: 487-99.
Doe, C. Q. (1992). Molecular markers for identified neuroblasts and ganglion mother cells in the Drosophila central nervous system. Development 116: 855-863.
Schmidt, H., Rickert, C., Bossing, T., Vef, O., Urban, J., and Technau, G. M. (1997). The embryonic Central Nervous System lineages of Drosophila melanogaster II. Neuroblast lineages derived from the dorsal part of the neurectoderm." Dev Biol 189: 186-204.